Stress is a phenomenon that we encounter more and more in often times. There is almost no person who has not experienced a stressful event in their lifetime. As we live in an age that lives fast so our work becomes more dynamic and demanding. Workers are required to do much longer, be more dedicated, have more knowledge and skills which leads to stress. In both business and private life, we are exposed to stress, which is unavoidable phenomenon nowadays. The graduate thesis consists of three main parts which deals with concept of stress, stress in business and stress management within the business. The aim of this graduate thesis is to show negative effect of stress on employees. The first part of the paper deal with the concept of stress, development of stress through history, sources of stress and types of stress by duration and strength. Furthermore, the notion of stress within the business and the impact of the business on the occurrences od stress ae addressed. The graduate thesis explains the sources of stress within a business that can be individual, group and organizational. There are various ways to prevent stress that can be undertaken by the individual himself to help him deal with stressful situations. The effects of stress can be various, of course negative both towards the employee and towards the organization. For an employee, as a result, burnout can occur by prolonged exposure to stress, while the company may deal with various costs and losses if it does not prevent stress between employees. The graduate thesis also explains the concept of stress as a modern disease that can affect very stressful occupations such as managerial occupations, and in modern times stress is also called managerial illness. The last part of the graduate thesis presents the ways of stress management in business, both individual and organizational ways. A great role in stress management is plays by the human resources department, which can help eliminate stress, but also make it worse if it does not do its job adequately.