The topic of the final paper is human resources management in family business. The main goal of the work is to explore and define the role and specificities of human resources management in family businesses compared to non-family businesses. This research topic is important because owners of family businesses face various difficulties in managing human resources, since the structure of employees consists also of family members and non-family members. In the theoretical part of the work, different definitions and typologies of family business are distinguished. A company, in the broadest sense, is defined as family when at least one or more family members are permanently employed in it. The activities of a family business are focused on investing and combining resources in order to achieve the income and goals of the business and the family. The second part of the paper focuses on the management of human resources, the specifics of human resources management in family businesses and the professional management in family businesses. It is well known how challenging it is to manage people, and for family business owners, it is an additional challenge to manage both family and non-family members employed in the company. As the basis of the empirical part of the work, a semi-structured interview with the owner of a family business was conducted. In the interview, the selected entrepreneur points out that it is challenging for him to balance business and family life and that this is stressful for him. He says that he tries to treat all employees equally and does not give benefits to employed family members so that other employees do not feel neglected. With all the responsibilities and obligations he has, he believes that he does not have enough time to dedicate himself to managing human resources and would like to delegate it to a professional manager. In the final part of the thesis, it is pointed out that family businesses have a shallow organizational structure and a small number of employees and do not have a special person or function dedicated to human resources. The management of human resources in a family business is therefore mostly the responsibility of the business owner.