Entrepreneurs have a key role in carrying out all entrepreneurial activities. Those are the ones who are ready to recognize new opportunities in the environment and find a way to use them. Except having an important role in the business world, entrepreneurs have an important role in everyday life. For that reason it is important to recognize and to understand what motivates and drives them. Although it is difficult to determine which specific traits successful entrepreneurs have, it can be said that they have a high level of self-awareness, internal locus of control, high need for achievement, are proactive and creative and take responsibility for their own actions as well as the risk that goes with it. As entrepreneurs live and do business in dynamic environment, it is important that an entrepreneurial ecosystem is developed and works supportive to their business. This paper consists two parts. The theoretical part of the paper presents and overview of the relevant literature on the meaning of entrepreneurship, entrepreneurial traits and entrepreneurial environment. As the practical part is based on the comparison of entrepreneurial traits and entrepreneurial environment in Osijek-Baranja and Dubrovnik-Neretva County, the theoretical part also contains a comparison of those two counties. The empirical part analyses the data gathered by primary research on a sample of 25 entrepreneurs from Osijek-Baranja and 25 entrepreneurs from Dubrovnik- Neretva County, who are doing business related to tourism. The data were analysed by descriptive and bivariate statistics. Based on conducted analysis, conclusions have been made on influence of entrepreneurial traits and environment and possible differences between two observed counties.