Through the study of entrepreneurship as a key component for economic development and growth in many countries around the world, the concept of the entrepreneurial ecosystem has developed with the aim of expanding the individual view to a multidimensional perspective including the role of social, cultural and economic forces. Networks of entrepreneurs, investors, support and support institutions, and other participants, within the entrepreneurial system, strive to meet the goals for creating an enabling environment for entrepreneurship and encouraging the development of entrepreneurial activities. The importance of entrepreneurship has been recognized globally, so in 1999 the Global Entrepreneurship Monitor (GEM) was launched as a research project operating in 100 countries, including Croatia. The aim of this research project is to explore the state of entrepreneurship in the world and Croatia and to identify best practices in encouraging and supporting entrepreneurship, and the indicator of the state of overall entrepreneurial activity, the TEA index, indicates the importance of research in this area. The paper explores the general goal of determining the state of the entrepreneurial ecosystem in Croatia, bearing in mind the consequences left by the COVID-19 pandemic with the specific goal of analyzing support institutions in Vukovar-Srijem County. Although Vukovar-Srijem County is facing a demographic decline, it has great potential. It has numerous raw materials of high quality and a large processing potential of primary production. The paper explores the specific objectives of the supporting institution in this area, such as: Local Development Agency Vjeverica d.o.o. and Agricultural Business Incubator Drenovci d.o.o..